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. 2016 Apr 13;49:23. doi: 10.1186/s40659-016-0082-7

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© Shabana and Hasnain. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Polyacrylamide gel electrophoresis of restriction digestion of amplified region for N103K, lane 1 is marker (25 bp DNA ladder SM#1191, Thermo Scientific), lanes 2 and 4 are heterozygous parents, lane 3 is homozygous proband, and lanes 5, 6 are controls. Presence of mutation abolishes one of the seven restriction sites of MnlI