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. Author manuscript; available in PMC: 2016 Apr 14.
Published in final edited form as: Biochem J. 2013 Mar 15;450(3):537–546. doi: 10.1042/BJ20121256

Figure 5. Co-immunoprecipitation assay of different SNARE complexes in mast cells.

Figure 5

Western blot analysis was performed using anti-SNAP-23 antibody after immunoprecipitating with several SNARE-specific antibodies. (b) Western blot analysis was performed using anti-Syn4 antibody after immunoprecipitating with several SNARE-specific antibodies. (c and d) Correlation between SNARE-mediated membrane fusion (lipid mixing assay) and SNARE complex formation in RBL-2H3 cells (co-immunoprecipitation). (e) Effect of polyphenols on the mRNA expression of SNARE genes in RBL-2H3 cells.