Table 1.
Antibody titer examined by IF after immunization of β3Gn-T5 KO mice
| Mouse (6102) | 10x | 30x | 90x | 270x | 810x | 2430x | |
|---|---|---|---|---|---|---|---|
| Pre-immune | K2 | - | - | - | - | - | - |
| ES2 | - | - | - | - | - | - | |
| SAOV | - | - | - | - | - | - | |
| Immunized x3 | K2 | 70 ++ |
70 + |
50 + |
20 ± |
- | - |
| ES2 | 90 ++ |
90 ++ |
70 + |
70 + |
50 + |
50 ± |
|
| SAOV | 80 ++ |
70 + |
40 + |
40 ± |
- | - | |
| Immunized x5 | K2 | 90 +++ |
70 ++ |
70 + |
40 + |
30 ± |
10 ± |
| ES2 | 100 +++ |
100 ++ |
90 ++ |
80 + |
80 + |
40 ± |
|
| SAOV | 100 +++ |
100 ++ |
80 + |
60 + |
40 + |
40 ± |
|
| Immunized x7(before fusion) | K2 | 90 +++ |
90 ++ |
80 + |
40 + |
40 ± |
- |
| ES2 | 100 +++ |
100 ++ |
90 ++ |
80 ++ |
80 + |
80 ± |
|
| SAOV | 100 +++ |
100 ++ |
90 ++ |
80 + |
40 + |
40 ± |
|
| Control (PC NC) | 50 ++ |
60 ++ |
50 ++ |
- | - | - | |
| K2 | ES2 | SAOV | K2 | ES2 | SAOV |
Results of IF with sera of a representative mouse (6102) were presented. Mice were immunized using glycolipids extracted from 3 ovarian cancer cells after embedding in liposome as described in MATERIALS AND METHODS. Numbers represent % of stained cells in IF, and grades of fluorescence intensity in positive cells were scored as -, ±, +, ++ and +++. Note increases in IF reaction with immunization times.