Fig. 9.

Expression of glucose transporters and transepithelial glucose transport by isolated pancreatic ducts. (A) mRNA expression of SGLT1, SGLT2, GLUT1, and GLUT2 in the entire pancreas (P), isolated interlobular pancreatic ducts (D), isolated pancreatic acini (A), small intestine (I), kidney (K), and lung (L) of the rat. (B) Absorption of luminal glucose. The lumen of the sealed ducts was micropunctured, and the luminal fluid was replaced with HEPES-buffered solution containing 44.4 mM glucose with or without phlorizin (0.5 mM). The bath was perfused with HEPES-buffered solution containing 44.4 mM glucose. Means ± S.E.M. (n = 4) of the time course changes in luminal volume. (C) Images of a duct at the beginning and end of a representative experiment.