Figure 3.

ERK5 inhibition reduces renal epithelial cell number. NRK cells transduced with lentiviral vectors expressing either control (Ctrl) or two distinct AGS3-specific shRNA (AGS3sh) were plated. NRK-Ctrl cells were counted in the presence of either vehicle (DMSO; ●), or two different doses of a selective ERK5/BMK1 inhibitor, BIX02189, at 3 (○) and 10 µM (▼). As a comparison, cell counts were determined for the NRK-cells expressing the AGS3 shRNA (NRK-AGS3sh; □). *P < 0.05 significant difference between vehicle (DMSO)-treated group. n = 3–6 experiments per time point.