Table 1.

Effects of LY379268 on the release of preloaded [³H]‐d‐Asp elicited by high KCl from cortical and spinal cord nerve endings from non‐immunized and EAE mice

	Control mice (induced overflow)	% of changes versus control	EAE mice (induced overflow)	% of changes versus control	% of changes versus EAE mice
Cortex 12 mM KCl	1.16 ± 0.05		0.75 ± 0.02 a	−35.34 ± 5.33
12 mM KCl/10 nM LY379268	0.63 ± 0.03 a	−45.68 ± 5.87 a	0.63 ± 0.09		−15.6 ± 4.11
Spinal cord 15 mM KCl	2.16 ± 0.06		3.07 ± 0.09 b	+42.12 ± 4.63
15 mM KCl/10 nM LY379268	1.37 ± 0.26 b	−36.57 ± 3.88	2.11 ± 0.07 c		−31.27 ± 3.15

Synaptosomes from control (non‐immunized) and from EAE mice (at 13 d.p.i. when studying then 12 mM KCl‐evoked release of [³H]‐d‐Asp from cortical nerve endings and at 21 d.p.i. when studying then 15 mM KCl‐evoked release of [³H]‐d‐Asp from spinal cord nerve endings) were exposed in superfusion to the depolarizing stimulus; 10 nM LY379268 was added concomitantly when indicated. Results are expressed as induced overflow as well as percentage of change versus control. Data are the mean ± SEM of at least four experiments run in triplicate (three superfusion chambers for each experimental condition).

^a P < 0.05 versus 12 mM KCl‐evoked [³H]‐d‐Asp from cortical nerve endings of non‐immunized mice.

^b P < 0.05 versus 15 mM KCl‐evoked [³H]‐d‐Asp from spinal cord nerve endings of non‐immunized mice.

^c P < 0.05 versus 15 mM KCl/10 nM LY379268‐evoked [³H]‐d‐Asp from cortical nerve endings of EAE mice.