Figure 5.

EP₁ receptor antagonism prevents AngII‐induced vascular stiffness and changes in the ECM. Representative fluorescent confocal photomicrographs of mPGES‐1 immunolocalization (A) (n = 5) and mRNA levels of mPGES‐1 (B) in aortic segments from mice with or without AngII treatment (n = 6). Image size 238 × 238 μm. (C) PGE₂ production by mesenteric arteries from mice with or without AngII treatment (n = 9) or AngII plus celecoxib (n = 10). (D) mRNA levels of mPGES‐1 in aortic homogenates from WKY (n = 10) and SHR (n = 11). Structural (E) and mechanical parameters (F) in mesenteric resistance arteries (MRA) from mice with or without AngII treatment (n = 5) or AngII plus SC19220 (n = 5). (G) Quantification of internal elastic lamina structure and collagen content in the media layer of MRA (n = 5). Elastin image size: 59.5 × 59.5 μm. Scale bar: 50 μm. Data represent mean ± SEM. Gene expression data are expressed as fold increase of the untreated or WKY groups mean value. *P < 0.05, significantly different from untreated. ^# P < 0.05, significantly different from AngII.