Figure 3.

Gβγ inhibition decreases STAT4 mRNA in TCR-stimulated CD4⁺ TH1 cells. (A) Gallein, but not fluorescein, significantly decreased STAT4 mRNA levels. Box plots (top) and difference plots (bottom) show data from naïve (left) and memory (right) CD4⁺ T cells isolated from the peripheral blood of 20 healthy donors, stimulated with plate-bound anti-CD3 and soluble anti-CD28, and grown in conditions promoting TH1 differentiation in the absence or presence of gallein or fluorescein for three days. (B) Gβ₁ siRNA significantly decreased STAT4 mRNA levels. Box plots (top) and difference plots (bottom) show data from primary human naïve (left) and memory (right) CD4⁺ T cells isolated from the blood of 30 healthy donors and stimulated for three days with plate-bound anti-CD3 and soluble anti-CD28 in conditions promoting TH1 differentiation in the presence of Gβ₁ siRNA or NT siRNA. STAT4 mRNA levels were determined by qPCR. ^*, p < 0.05; ^***, p < 0.001; ^****, p < 0.0001.