Figure 5.

Gβγ inhibition increases levels of mRNA encoding TH2 cytokines in TCR-stimulated CD4⁺ TH2 memory cells. (A) Gallein, but not fluorescein, significantly increased levels of IL-4, IL-5, IL-9, and IL-13 mRNA in TCR-stimulated TH2 memory cells. (B) Gallein, but not fluorescein, significantly increased IL-9, but not IL-4, IL-5, or IL-13 mRNA levels in TCR-stimulated TH2 naive cells. (C) Gβ₁ siRNA significantly increased IL-4, but not IL-5, IL-9, or IL-13 mRNA levels in TCR-stimulated TH2 memory cells. (A-B) Box plots (top) and difference plots (bottom) show data from memory (A) and naive (B) CD4⁺ T cells isolated from the peripheral blood of 20 healthy donors, stimulated with plate-bound anti-CD3 and soluble anti-CD28, and grown in conditions promoting TH2 differentiation in the absence or presence of gallein or fluorescein for three days. (C) Box plots (top) and difference plots (bottom) show data from memory CD4⁺ T cells isolated from the blood of 30 healthy donors and stimulated for three days with plate-bound anti-CD3 and soluble anti-CD28 in conditions promoting TH2 differentiation in the presence of Gβ₁ siRNA or NT siRNA. mRNA levels were determined by qPCR. ^*, p < 0.05; ^**, p < 0.01; ^***, p < 0.001.