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. 2016 Apr 14;4(7):e12749. doi: 10.14814/phy2.12749

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Laser speckle imaging. Experimental set‐up (A) and representative images (B) using full‐field LSI to determine hemispheric blood flow (measured in arbitrary units of “flux”). The lower panel shows six animals with scalps exposed, within the hypoxia chamber. Animals faced in alternating directions to minimize breathing restrictions. Cerebral blood flux measurements (1) after ligation; (2) during hypoxia; and (3) after 10 min of resuscitation in 21% oxygen are shown. Representative bilateral regions of interest over the ligated (L) and unligated (R) hemispheres are shown in the animal on the right.