Figure 7.

Expression of CRMP4 mRNA and MAP2 in HT22 cells. (A) HT22 cells express CRMP4 mRNA, whereas transfection with CRMP4 siRNA (CRMP4‐KD) significantly decreased its expression. (B) (Upper) Representative micrograph of HT22 cells immunohistochemically stained with an MAP2 antibody (green). (Bottom) Differential interference contrast image of the same cells. Neurites of HT22 cells show immunoreactivity to MAP2. Bars in (A) indicate mean ± SE. Student's t‐tests were performed to compare the means, ***P < 0.001. Scale bar: 50 μm.