Figure 3.

The effect of ERK1/2, p38 MAPK and AKT inhibitors on IL‐1β‐induced CCL20 production in A549 cells. Cells were pre‐treated with or without inhibitors of ERK1/2 (PD98059), p38‐MAPK (SB202190) and AKT (SHBM1009) for 1 hr and stimulated with IL‐1β (1 ng/ml), and the total mRNA was harvested in 4 hrs for RT‐PCR analysis (A) or the conditioned medium was collected in 24 hrs for ELISA assay (B). ** stands for P‐values less than 0.05 and 0.01, as compared with cells only with DMSO, and ++ stands for P‐value less than 0.05 and 0.01, as compared with IL‐1β and DMSO respectively. Data were presented as mean ± S.E. and each group has at least six measurements.