Figure 4.

Ovariectomy enhances liver regeneration. (A) Ovariectomy reduces the levels of the miR‐17~92 cluster members in WT mice. (B) Oestrogen (E2) stimulation promotes miR‐17~92 production in cultured HepG2 cells. (C) Liver regeneration is promoted in female mice with both genotypes in response to ovariectomy, as determined by the number of Ki67‐positive hepatocytes. (D and E) Both Ki67 immunoreactivity and BrdU incorporation indicate that there is no difference in the regeneration rate between the WT and CKO mice after ovariectomy. *P < 0.05, scale bar: 50 μm.