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. 2016 Feb 9;20(5):855–863. doi: 10.1111/jcmm.12790

Figure 4.

Figure 4

VCAM‐1 staining in aortic sinus from mice fed an HD or standard chow diet. (A) Representative histological sections of the aortic sinus stained with blue‐haematoxylin for VCAM‐1 in NI mice fed a standard chow diet or an HD and in immunized mice fed a standard diet (CIA) or an HD (CIA+HD). Magnification is 50 × (a, d, g, j) or 400 × (b, e, h, k). Staining for isotype control of VCAM‐1 antibody (IgG2a, κ antibody) is shown in c, f, i and l (×400 magnification). Black arrows show VCAM‐1 endothelial staining; white arrows show VCAM‐1 staining in the aortic sinus wall. (B) Quantification of VCAM‐1–positive staining by use of Image J Fiji. $ P < 0.05 versus NI.