Figure 3.

The influence of temperature on the centromere of Xanthomonas citri subsp. citri (Xcc). The Xcc parB::pAPU3 mutant, expressing ParB‐GFP, was cultivated in NYG‐medium at 30°C until the OD _600 nm of ~0.4; cultures were transferred to different temperatures as indicated, and thermal stress was carried out for a period of 6 h. Cells were visualized immediately after the shifts. Panels: A–B) 30°C, C–D) 20°C, E–F) 37°C, G–H) 40°C, and I–J) 42°C; (PhC) phase contrast microscopy and (GFP, green fluorescent protein) fluorescence microscopy. Magnification 100×; bar = 4 μm.