Figure 1. IFN-γ KO mice generate CTL responses following s.c. immunization, yet the CTL responses do not provide protection against subsequent tumor challenge in the eye.

CTL was isolated from the spleens 14 d following s.c. immunization of WT and IFN-γ KO mice with Ad5E1 tumor cells. (A) Both WT and IFN-γ KO mice developed CTL responses following s.c. immunization. (B) Subcutaneous immunization before AC challenge does not protect against AC tumor growth in IFN-γ KO mice but is protective in the WT mice. (C) Subcutaneous immunization before AC challenge induces peripheral CTL responses in both WT and IFN-γ KO mice, and cytolytic function is retained. Three different effector to target ratios (E:T) were tested. Results are expressed as mean percent cytolysis from 4 replicates. *<1% cytolysis. Data are representative of 2 independent experiments with 5 mice per group.