Figure 10. Increased Th cell–associated CD4 message distinguishes adoptive transfer (AT) recipient IFN-γ KO mice that pristinely rejected their intraocular tumor when compared with those that developed progressive intraocular tumors.

Eyes were removed from WT and IFN-γ KO mice 24 d after the mice received AT CD8⁺ T cells from s.c.-immunized WT donors and were challenged in the AC with Ad5E1 tumor cells. Eyes were examined by qPCR for expression of the following immune cell phenotype genes: (A) CD3 (CD3e), (B) CD4 (CD4), (C) CD8 (CD8a), (D) CD19 (CD19),(E) CD68 (CD68), and (F) CD11c (itgax). Log₂-transformed means of 5 biologic replicates in each of the 5 groups are shown with sds. The least-expressing sample (including naïve animals) was used as the baseline for each gene assayed. Results are representative of 2 independent qPCR experiments. There were 5 mice per group. Groups were compared using the Student’s t test, and P < 0.05 is shown.