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. Author manuscript; available in PMC: 2016 Apr 14.
Published in final edited form as: J Pharmacol Toxicol Methods. 2015 Aug 20;76:76–82. doi: 10.1016/j.vascn.2015.08.154

Table 1.

Sau3A1 enzyme digestion reaction setup.

Component Quantity/tube (µl)
CPF-resistant cellular DNA 2 µg calculations (Fig. 2)
Sau3A1 enzyme (20 U/µl) 2
10× NEBuffer 1 3
100× BSA (10 mg/ml) 1
Double-distilled water Bring final volume to 30
Final volume 30