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. Author manuscript; available in PMC: 2016 Apr 14.
Published in final edited form as: J Pharmacol Toxicol Methods. 2015 Aug 20;76:76–82. doi: 10.1016/j.vascn.2015.08.154

Table 2.

Adaptor ligation.

Component Quantity/tube (µl)
Sau3A1 digested DNA 4.5
Adaptor mix (25 µM) 1
T4 DNA ligase (20 U/µl) 1
10× T4 DNA ligase buffer 4
Double-distilled water 29.5
Final volume 40

Note: Ligation reaction setup, utilizing a distinct adaptor mix with T4DNA ligase. Required quantities per 40 µl total reaction volume.