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. Author manuscript; available in PMC: 2016 Apr 14.
Published in final edited form as: J Pharmacol Toxicol Methods. 2015 Aug 20;76:76–82. doi: 10.1016/j.vascn.2015.08.154

Table 3.

Nhe1 enzyme digestion.

Component Quantity/tube (µl)
Adaptor ligated DNA 40
10× NEBuffer 2 10
100× BSA 1
Nhe1 (10 U/µl) 2
Double-distilled water 47
Final volume 100

Note: Nhe1 enzyme digestion reaction setup. Required quantities per 100 µl total reaction volume.