Table 4.
Oligonucleotides and primers used.
| Sequence (5′–3′) | |
|---|---|
| Adaptor ligation | |
| Short-Strand Adaptor |
GATCCCACTAGTGTCGACACCAGTCTCTAATTTTTTTTTTCAAAAAAA |
| Long-Strand Adaptor |
GAAGAGTAACCGTTGCTAGGAGAGACCGTGGCTGAATGAGACTGGT GTCGACACTAGTGG |
| Splinkerette PCR | |
| Splink1 | GAAGAGTAACCGTTGCTAGGAGAGACC |
| U3LTR#5 | GCGTTACTTAAGCTAGCTTGCCAAACCTAC |
| Splink2 | GTGGCTGAATGAGACTGGTGTCGAC |
| U3LTR#1 | CCAAACCTACAGGTGGGGTCTTTC |
| qRT-PCR | |
| ATP8B2-F | GGGTGCTAACCGGAGAC |
| ATP8B2-R | AGTATGGCCAGTGACTATGAA |
| DCAF12-F | CTTGCCATCTATCGACTACCT |
| DCAF12-R | CCATAGTGTCGCTGATCCA |
| FNBP4-F | TGGCAGGAGTCGGAATTGAG |
| FNBP4-R | ACCTGTGTGGCAAGATATTGG |
| LAT2-F | GACCAACAGAGCTTTACGG |
| LAT2-R | CTGGGGTAGAATTGC |
| BIK-F | GGTTCTTGGCATGACTGA |
| BIK-R | GGCCAATGCGTCACT |
| AGPAT6-F | GGCAGGGAGGATTGGTAG |
| AGPAT6-F | TCATGGTTTCCGACTATCAT |
| AIG-F | CGCACCATCAGTATCCC |
| AIG-R | ACACCCACATGCCAGTTACA |
| PTC7-F | AAACCTGGGCGATTC |
| PTC7-R | ATCCGGACTGTCGCTCAAGA |
| GAPDH-F | ACATCGCTCAGACACCATG |
| GAPDH-R | TGTAGTTGAGGTCAATGAAGGG |