Figure 4.

PMA-induced NETs activate complement to form C5b–9. Neutrophils were left untreated (A,D,G), activated with 20 nM PMA (B,E,H), and treated with DNase I after PMA activation to disseminate the formation of NETs (C,F,I). After induction of NETosis in RPMI buffer, 20% (v/v) autologous plasma was added. (A–C) NETs were visualized with DAPI. (D–F) C5b–9 was detected using mouse monoclonal antibody to C5b–9. Image was taken with 60×/1.35 oil immersion objective. (H) with 2× magnified inset of representative area. Scale bar, 9.00 μm.