Fig 6. A sublethal infection primes bone marrow and blood phagocytes to increased Dectin-1 and CXCR2 expression.

To investigate the priming process following the subethal infection, mice were either sacrificed at day 0 (Naïve) or at day 10 following infection with the sublethal dose (Primed). (A) CXCR2 expression on bone marrow and blood neutrophils (GR1^high CD11b⁺ population). Dectin-1 expression levels on bone marrow and blood neutrophils (GR1^high CD11b⁺ population) (B), and macrophages (F4/80⁺CD11b⁺GR-1^- population) (C). (D) Dectin-1 expression levels on BAL neutrophils and macrophages. Representative histograms show level of expression (left panel) and quantification of respective values in graphs (right panel). (E) Dynamic of Dectin-1 level expression on both neutrophils (left panel) and macrophages (right panel) in the BALs in the three infection settings. “SL” or “L” mice were sacrificed at 0 h (naïve), 12 18, 24, 48, 72 and 144 h p.i. and “Re-inf” mice were sacrificed at 0 h (10 days after the SL infection, day of the re-infection), 12, 18,24, 48, 72 and 144 h p.i. Data (mean ± SEM) represent 2 to 3 independent experiment with n = 5 mice per experiment. Statistically significant differences were determined using a Two way ANOVA with Bonferroni post-test (+p<0.05, ++p<0.01; *p<0.05, **p<0.01, ***p<0.001Re-Inf vs SL dose; #p<0.05, ##p<0.005 ###p<0.001 LL dose vs Re-Inf mice).