Fig. 2.

Murine gamma herpes virus 68 (γHV68) transiently alters core body temperature and wheel running activity of C57BL/6J mice. Mice were inoculated intranasally with Hank’s balanced salt solution containing either 400 plaque forming units (pfu) of γHV68 (n = 13) or 40,000 pfu (n = 8) according to the protocol detailed in Fig. 1. Values (mean ± SEM) are depicted for the 12 h dark period (filled circles) and the 12 h light period (open circles) for 28 consecutive days after intranasal inoculation with virus. The horizontal lines on each panel represent the mean dark or light phase values obtained during the 4 day baseline recording period (protocol days −4 to 0). Data for wheel running activity from the 40,000 pfu group are missing from the dark period of day 26 due to computer failure. The vertical dashed lines on the panels depicting data from the 40,000 pfu group demarcate post-inoculation days 8 and 9. Responses to γHV68 on post-inoculation days 8 and 9 are presented in detail in Fig. 3. Horizontal bars with asterisks indicate statistically significant differences from control values (p < 0.05).