Table 5. Genes involved in pollen-pistil interaction.

Gene	Biological Process	Tissue	References	BLAST best matcha	EST IDb	Length (bp)	E-valuec
Lipid transfer protein precursor (LTP)	Adhesion of pollen tubes to transmitting tissue	Pistil	[26, 55]	[L. longiflorum] AF171094	unigene05516	841	3e-26
					unigene08514*	610	6e-33
					unigene07617*	669	1e-30
					unigene07897*	648	1e-30
					unigene07066*	708	6e-29
Pollen-specific gene (LAT52)	Hydration and pollen tube growth	Pollen	[26, 56]	[S. lycopersicum] CAA33854.1	unigene06374*	764	2e-53 2e-051 1e-028 5e-028 8e-028
					unigene06415	762	2e-051
					unigene07787	658	1e-028
					unigene06119	785	5e-028
					unigene05934	802	8e-028
					unigene05528	829	9e-028
					unigene05190	862	8e-027
					unigene06002	797	9e-027
					unigene05771	816	2e-026
					unigene06255	777	2e-026
					unigene05964	800	5e-026
					unigene05896	810	8e-026
					unigene06066	794	1e-025
					unigene07219	684	3e-024
					unigene06532	752	3e-023
Pollen-specific gene (NTP101)	Pollen tube growth	Pollen	[57]	[N. tabacum] AAQ90182.1	unigene03925*	991	1e-127
Pectin methylesterase (PME)	Pollen tube growth	Pollen	[58]	[N.acum] AAX13972.1	unigene00539	1,811	1e-170
					unigene00473	1,867	1e-169
					unigene01185*	1,493	6e-041
					unigene01763*	1,317	5e-039
Oleosin (OLE)	Pollen hydration	Pollen	[26]	[C. oleifera] ABF57563.1	unigene08062*	638	3e-31
Beta-1,3glucanase (βGLU)	Callose degradation in the process of tetrad dissolution	Anther	[59]	[O. sativa] BAC02926.1	unigene00872*	1,632	3e-056

Olive candidate genes that are putatively involved in pollen-pistil interactions. The genes were confirmed by RT-qPCR experiments.

^aSpecies and accession number of the genes that were used as reference sequences for tBLASTx search.

^bBest hits of the olive transcripts, as identified by a bi-directional BLAST search.

^cE-value of the best hit identified by the BLASTX search.

*Transcripts selected for the RT-qPCR analyses.