Fig 1. Transcriptome and translatome profiling for YJM789 and BY4742.

(A) Workflow for the experimental procedures. The two yeast strains were cultured in YPD to log-phase. Cells were collected and treated with cycloheximide (CHX) for two minutes, and then divided into two aliquots, one for RNA-Seq and the other for ribosomal footprint profiling. Raw sequencing reads were cleaned and mapped to the yeast genomes for further analyses. (B) Spearman correlation coefficient (ρ) among mRNA and RFP data between biological replicates (indicated by numbers) and strains (indicated by letter, B: BY4742 and Y: YJM789). (C) Improving the YJM789 annotation using both mRNA and RFP data. Representative cases are depicted for three types of modifications: missing ORF (YIR019C), ORF extension (YEL046C) and ORF trimming (YER050C).