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. 2016 Apr 14;11(4):e0153513. doi: 10.1371/journal.pone.0153513

Fig 1. Zinc availability regulates the lifespan of C. elegans.

Fig 1

(A) Kaplan-Meier survival curve of worm populations exposed to zinc or TPEN as larvae. Wildtype worms were exposed to 500μM ZnSO4 or 200μM TPEN at the L3 stage. Mean and maximum population lifespan were reduced with zinc and increased with TPEN treatment (p < 0.0001, log rank test) (B) Kaplan-Meier survival curve of worm populations exposed to zinc or TPEN as adults. Wildtype worms were exposed to 500μM ZnSO4 or 200μM TPEN at day 5 of adulthood. Thl.ere was no significant difference in mean and maximum population lifespan due to zinc or TPEN treatment. (C) Changes in total zinc content in worms exposed to zinc or TPEN as larvae. Wildtype worms were exposed to 500μM ZnSO4 or 200μM TPEN at the L3 stage and collected at one-day old adult animals for analysis of total zinc content. Worms supplemented with zinc had a ~2-fold increase in total zinc content (***, p<0.0001, t-test), while worms treated with TPEN demonstrated a ~2-fold decrease in total zinc content (**, p<0.001, t-test). Data shown are the mean ± SD of 3 experimental replicates.