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. 2016 Apr 14;11(4):e0153886. doi: 10.1371/journal.pone.0153886

Fig 1. Increased glycolytic metabolism during osteoclast differentiation.

Fig 1

Osteoclast precursors were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for the indicated times. (A) Glucose and lactate contents in cell culture media. Concentrations of glucose and lactate in the culture medium were measured. Data are given as mean ± SD for a representative experiment run in triplicate. *P < 0.01, **P < 0.05. (B) Gene expression analysis of monocarboxylate transporters (MCTs) during osteoclast differentiation. Total RNA isolated from cells was subjected to RT-PCR analysis of the indicated mRNAs. Level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as an internal control for equal loading.