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. 2016 Apr 14;11(4):e0153568. doi: 10.1371/journal.pone.0153568

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© 2016 Cury et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Light microscopy of bone-tendon junction of the calcaneal tendon of adults Wistar rats (A, B, C, D, E, C’, D’, and E’) and elderly Wistar rats (F, G, H, I, J, H’, I’, and J’). (A, B, C, D) Calcaneal tendon (T), periosteal fibrocartilage (PF), trabecular space (*), trabecular bone (arrows). Stain: Fig A: Picro-Sirius. Fig C: Picro-Sirius under polarized light. Bars: 50 μm, x100. (C’) Calcified fibrocartilage (CF), rows of cells (arrows), bone (B). Stain: Picro-Sirius. Bar: 20 μm, x200. (D’) Calcified fibrocartilage (CF), rows of cells (arrows), bone (B), division between the collagen fibers of the tendon and bone (dashed line). Stain: Picro-Sirius under polarized light. Bar: 20 μm, x200. (E) Uncalcified fibrocartilage (UF), calcified fibrocartilage (CF), tidemark (dashed ellipse), trabecular space (*), trabecular bone (arrow). Stain: Hematoxylin–eosin. Bar: 50 μm, x100. (E’) Fibrocartilage cells in the uncalcified fibrocartilage (arrowhead), fibrocartilage cells in the calcified fibrocartilage (larger arrows), tidemark (small arrows). Stain: Hematoxylin–eosin. Bar: 20 μm, x200. (F, H) Calcaneal tendon (T), trabecular space (arrowhead), trabecular bone (arrow), periosteal fibrocartilage (PF). Stain: Picro-Sirius. Bar: 50 μm, x100. (G, I) Calcaneal tendon (T), trabecular bone (arrows), periosteal fibrocartilage (PF). Stain: Picro-Sirius under polarized light. Bar: 50 μm, x100. (H’) Calcified fibrocartilage (CF), rows of cells (arrows), bone (B). Stain: Picro-Sirius. Bar: 20 μm, x200. (I’) Calcified fibrocartilage (CF), bone (B), division between the collagen fibers of the tendon and bone (dashed line). Stain: Picro-Sirius under polarized light. Bar: 20 μm, x200. (J) Uncalcified fibrocartilage (UF), calcified fibrocartilage (CF), tidemark (dashed ellipse), bone (*). Stain: Hematoxylin–eosin. Bar: 50 μm, x100. (J’) Fibrocartilage cells in the uncalcified fibrocartilage (arrowhead), fibrocartilage cells in the calcified fibrocartilage (larger arrows), tidemark (small arrows). Stain: Hematoxylin–eosin. Bar: 20 μm, x200.