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. 2016 Apr 14;12(4):e1005523. doi: 10.1371/journal.ppat.1005523

Fig 6. Co-expression of the hPBV RdRP and CP.

Fig 6

(a) TEM images of the purified hPBV VLPs for the WT CP (top) and the Δ45CP (bottom). The scale bar represents 50 nm. (b) SDS-PAGE analysis of the recombinant capsids of both the full-length and Δ45 CPs. (c) Co-expression of the hPBV CP and RdRP. Samples included are a prestained protein marker (lane 1), the soluble fraction of the cell lysate (lane 2), the Ni-NTA bound fraction (lane 3), and the purified VLPs (lane 4). Proteins were separated by SDS-PAGE and detected by Western blot using either anti-6xHis or anti-CP antibodies (two upper panels) or staining with Coomassie blue (lower panel). The molecular weights of the pre-stained marker proteins are indicated in kDa on the left.