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. 2016 Apr 14;12(4):e1005523. doi: 10.1371/journal.ppat.1005523

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© 2016 Collier et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — RNA binding by the hPBV WT (a, c) and ΔLOOP (b, d) RdRPs analyzed by gel shift assays. The fraction of RNA bound was quantitated and plotted as a function of protein concentration. The values were normalized by the total amount of RNA. (a, c) Three distinct RNA oligonucleotides were used, including: (1) the first 20 nucleotides of the 5’-(+)UTR of hPBV genome segment 2 (black); (2) the last 20 nucleotides of the 3’-(+)UTR of the hPBV genome segment 2 (red); and (3) a 20-mer nonsensical CA repeat (blue). (b, d) Two RNA molecules derived from the PBV2+ were used, including the full-length PBV2+ and the PBV2+(Δ1–645). The obtained dissociation constants (K_d) for the different RNA oligonucleotides are indicated on the right.