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. 2016 Apr 15;27(8):1210–1219. doi: 10.1091/mbc.E15-10-0697

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© 2016 Gowda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Fes1S is a cytosolic protein, and Fes1L is targeted to the nucleus by a C-terminal NLS. (A) An NLS-like sequence in the C-terminus of Fes1L and the changes in amino acid sequence of the Fes1L mutant K/R → E. (B) Western analysis of C-terminally GFP-tagged Fes1S, Fes1L, and K/R → E expressed from plasmids with the endogenous FES1 promoter in fes1Δ cells. (C) Fluorescence microscopy localization of the GFP fusions in B. The nucleus is labeled with mCherry-tagged Histone 2B (Htb2). Scale bar, 10 μM.