FIGURE 6:

Fes1S is essential for ubiquitin-proteasome system–dependent degradation of misfolded proteins. (A) Western analysis of the turnover of the misfolded model protein Rpo41* after the addition of cycloheximide (CHX). Strains as in Figure 4A. Error bars represent SD (N = 3). (B) Western analysis as in A but of misfolded model protein Ste6* C. (C) Western analysis as in A but of the turnover of the nuclear-targeted misfolded model protein ΔssPrA. (D) Cell suspensions of the strains expressing empty vector control (VC) or Ste6* C or ΔssPrA were serially diluted 10-fold and spotted onto selective medium. Photographs were taken after 3 d of incubation at 25°C. Bottom, Western analysis of the steady-state expression of ΔssPrA.