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. 2016 Apr 15;27(8):1235–1245. doi: 10.1091/mbc.E15-10-0700

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© 2016 Wei et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Gef1 promotes type V myosin Myo52 localization to the cell division site. (A) Type V myosin Myo52-GFP distribution in gef1⁺ and gef1Δ cells. Cells with comparable distance of SPB marker Sad1-mCherry were selected. Bright-field images ensured nonseptating cells were selected. Red arrows mark cytoplasmic Myo52-GFP patches. Asterisks mark cells in the ring phase of cytokinesis. (B) Quantification of Myo52-GFP signal in the cytoplasm in gef1⁺ and gef1Δ cells as described; 22 cells, **p = 0.0015. Error bars, SD. (C) Calcofluor staining of gef1⁺for3⁺, gef1Δ, for3Δ, and gef1Δfor3Δ cells grown at 35°C. Bars, 5 μm.