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. 2016 Apr 15;27(8):1235–1245. doi: 10.1091/mbc.E15-10-0700

FIGURE 7:

FIGURE 7:

Gef1 promotes recruitment of Bgs1 but not Bgs4 to the site of cell division. Localization of septum protein (A) Bgs1-GFP and (B) Bgs4-RFP at the site of cell division. Time 0 represents SPB separation. Red box, onset of Bgs1-GFP or Bgs4-RFP recruitment to the site of cell division. Green box, appearance of Bgs1-GFP or Bgs4-RFP as a ring at the division site. Bars, 5 μm. (C) Quantification of time of recruitment of Bgs1-GFP with reference to cytokinetic ring formation as depicted by Rlc1-Tomato; >14 cells. Time in minutes. ***p < 0.001. Error bars, SD. (D) Quantification of time of recruitment of Bgs4-RFP with reference to SPB separation as depicted by Cdc11-GFP; 21 cells. Time in minutes. ns, not significant; **p < 0.01. Error bars, SD.