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. 2016 Apr 15;27(8):1246–1261. doi: 10.1091/mbc.E15-08-0565

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© 2016 Monzo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — GFP-FHOD3 localizes on parallel actin filaments found in leading processes. C6 glioma cells were transfected with GFP-FHOD3 and seeded on 20-μm laminin micropatterned lines (A) and 2D substrates (B). Cells were fixed and stained with phalloidin and 4′,6-diamidino-2-phenylindole and imaged using a confocal microscope. (B) GFP-FHOD3–overexpressing cells (asterisks) among untransfected cells display excessive amounts of longitudinal actin bundles. Scale bars, 10 μm.