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. 2016 Apr 15;27(8):1272–1285. doi: 10.1091/mbc.E15-08-0561

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© 2016 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — The effect of POML-1 on MEC-4(d) channel activity and their physical interaction in Xenopus oocytes. (A) Effect of POML-1 (white bars) on the MEC-4(d) amiloride-sensitive current at −85 mV in oocytes. The number of oocytes tested is given in parentheses. The oocytes were from at least two frogs. (B) Immunoprecipitation (IP) of POML-1 with MEC-4(d) and MEC-6 expressed in oocytes. IB, immunoblot probe. Images are representative of two or three independent experiments. Molecular weights (kilodaltons) of the protein markers are indicated on the right. EGFP::POML-1 is functional, since its coexpression with MEC-4(d) generated amiloride-sensitive currents (EGFP::POML-1 and Myc::MEC-4(d), I_amil = −1.4 ± 0.3 μA, n = 5) that were similar to those of coexpressing untagged POML-1 and MEC-4(d) (I_amil = −1.6 ± 0.6 μA, n = 5) 6 d after cRNA injection. The negative control (–) is EGFP with the same tag.