FIGURE 1:

Design of a system for reversible masking of transport signals. (A) Scheme of the SBP-based constructs. The two amino acid triplets of SBP that interact with the biotin-binding sites of SA (Barrette-Ng et al., 2013) are underlined. (B) Schematic representation of the CUTE approach. When targeting signals are appended to SBP, binding of SA may hinder the signals and thus prevent transport to the target compartment. Biotin-induced SA dissociation from SBP would then lead to unmasking of the signal, followed by synchronous transport.