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Effect of the insertion of linkers between SBP and the targeting signals. Cells were transfected with the indicated constructs with or without streptavidin and incubated for 24 h at 37°C; for CD4 constructs, cells were permeabilized and stained with anti-CD4 antibodies. lnk, GGGGSGGGGS; lnkx2, GGGGSGGGGSGGGGSGGGGS.
