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. 2016 Apr 15;27(8):1310–1319. doi: 10.1091/mbc.E15-07-0472

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© 2016 Abraham, Gotliv, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Masking/unmasking of a signal for peroxisomal import. (A) GFP-SBP-SKL localizes to peroxisomes. GFP-SBP-SKL was cotransfected with the peroxisomal marker RFP-SKL. GFP-SBP (without SKL) served as a control. (B) Biotin-dependent import into peroxisomes. GFP-SBP-SKL was cotransfected with SA (plasmid ratio, 1:10) and cells were incubated for 4 h with or without biotin in the presence of cycloheximide. (C) Live imaging of biotin-induced peroxisomal import. Images were taken from Supplemental Movie S2.