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. 2016 Apr 15;27(8):1310–1319. doi: 10.1091/mbc.E15-07-0472

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© 2016 Abraham, Gotliv, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Golgi-to-ER retrieval of VSVGts045 followed with a photoconvertible fluorescent protein. Cells were cotransfected with VSVGts045-dendra2-SBP-KRKAE and SA. After overnight incubation at 40°C, the temperature was shifted to 32°C for 1 h to allow the VSVGts045 construct to reach the Golgi. The dendra2 protein was then photoconverted at the Golgi area, and the cells were followed by live imaging at 32°C. Biotin was added immediately before photoconversion (bottom lane). The images marked 3 h* were taken after refocusing for better clarity.