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. 2016 Apr 15;27(8):1310–1319. doi: 10.1091/mbc.E15-07-0472

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© 2016 Abraham, Gotliv, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — Role of the Golgi apparatus in PM-to-ER transport of CD4_1–429-SBP_EL-KRKAE. (A) CD4_1–429-SBP_EL-KRKAE partially colocalizes with Golgi and endosomal markers after 1 h of biotin treatment. Organelle markers GalT-CFP, GFP-ManII, and GFP-EEA1 were introduced by plasmid transfection, and TGN was detected using anti-TGN46 antibodies. (B) BFA (0.2 μg/ml) and GCA (5 μg/ml) cause the dispersal of the Golgi marker GalT into the ER after 4 h of incubation. NT, nontreated. (C) BFA and GCA block biotin-induced transfer of CD4_1–429-SBP_EL-KRKAE to the ER. Cells were incubated with biotin for 4 h in the presence or absence of BFA or GCA and were processed as in Figure 8A.