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. 2016 Apr 15;27(8):1358–1366. doi: 10.1091/mbc.E15-10-0738

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© 2016 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — VEGF attenuates H₂O₂-induced ASK1 activation. HUVECs were treated with different concentrations of H₂O₂ for 24 h. Cell viability (A), phosphorylation of ASK1 at Ser-83 (B), and methylation of ASK1 (C, D) were determined by the indicated antibodies. HUVECs were pretreated with VEGF for 30 min and then treated with 300 μm H₂O₂ for 24 h. Cell viability (E) and phosphorylation of ASK1at Ser-83, cleaved caspase-3 (F) and methylation of ASK1 (G) were determined by the indicated antibodies. *p < 0.05 compared with control group.