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. 2016 Apr 15;27(8):1383–1396. doi: 10.1091/mbc.E14-05-1013

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© 2016 Airoldi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Growth rate response of gene expression in steady-state and dynamic conditions. (A) We estimated the growth rate response of each transcript in steady-state and dynamic conditions by linear regression. In dynamic conditions in which nitrogen limitation is transiently relieved, the mRNAs belonging to the RiBi and RP regulons exhibit a growth rate response that exceeds that observed in steady-state chemostats. High temporal resolution shows a reciprocal relationship between expression of the RiBi and RP regulons, which increase in expression, and the NCR-A and NCR-P regulons, which decrease in expression, in response to a (B) 40 μM glutamine pulse and a (C) 80 μM proline pulse. Time points (in seconds) at which samples were obtained and analyzed are indicated on the x-axis.