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. 2016 Apr 12;7:11277. doi: 10.1038/ncomms11277

Figure 9. Deletion of HDAC4 partially recapitulates the PPR-deficient root phenotype.

Figure 9

(a–c) Mandibular first molar (M1) sections of (a) HDAC4+/+ (WT), (b) HDAC4+/− (HT) and (b) HDAC4−/− (KO) at P10 were stained for hematoxylin and eosin. Lower panels: magnified views of dotted areas. Yellow arrowheads: embedded cementocytes. Scale bars: 500 μm (upper panels) and 50 μm (lower panels). (d,e) Mandibular first molar (M1) sections of (a) Control (HDAC4fl/fl) and (b) Osx-HDAC4 conditional KO (Osx-cre; HDAC4fl/fl) mice at P25 were stained for hematoxylin and eosin. Lower panels: magnified views of the dotted areas. Yellow arrowheads: thick and irregular cementum. Scale bars: 500 μm (upper panels) and 50 μm (lower panels). (f) Shown is quantification of M1 distal root length at P25. Blue bars: Control, red bars: Osx-HDAC4 conditional KO. n=4 per group. (g,h) Sections of (g) Control (HDAC4fl/fl) and (h) Osx-HDAC4 conditional KO (Osx-cre; HDAC4fl/fl) mice at P7 were stained for nuclei and EdU. EdU was administered twice (6 and 3 h) prior to analysis. Red: EdU-Alexa555, grey: DAPI. Yellow dotted lines: HERS. Scale bars: 50 μm. (i,j) Shown is quantification of EdU+ cells in dental papilla (i) and dental follicle (j) around HERS at P7. Blue bars: Control, red bars: Osx-HDAC4 conditional KO, n=4 per group, *P<0.05, Mann–Whitney's U-test. All data are represented as mean±s.d.