Figure 3.

Autophagy is dispensable for B-cell development. Bone marrow cells from one femur of each wild-type C57BL/6 mice (B6), Atg5^f/+ CD21 cre (littermates CD21 cre) or Atg5^f/+ Mb1 cre (littermates Mb1 cre), Atg5^f/− CD21 cre (CD21 cre) or Atg5^f/− Mb1 cre (Mb1 cre) were stained with anti-IgM, anti-B220, and anti-CD43 Abs and analysed by flow cytometry. (a) Representative dot plots of FSC and SSC profiles of bone marrow cells. (b) Individual values for each mouse tested of absolute B220+ cell numbers, in the bone marrow obtained from B6 mice n=4, littermate CD21 cre mice n=3, littermate Mb1 cre mice n=5, CD21 cre mice n=5, and Mb1 cre mice n=5. Means and standard deviations (S.D.) are indicated. (c) Representative dot plots for the expression of B220 and surface IgM, allowing definition of pre-/pro-B cells (IgM⁻B220^lo), immature B cells (IgM⁺B220^lo), and mature B cells (IgM⁺B220^hi). (d) Individual values for each mouse tested of percentages of different B-cell precursors populations depicted in (c), in bone marrows obtained from B6 mice n=5, littermate CD21 cre mice n=4, littermate Mb1 cre mice n=7, CD21 cre mice n=4, and Mb1 cre mice n=5. Means and S.D. are indicated. (e) Representative dot plots obtained by flow-cytometry analysis, showing the percentages among B220⁺ cells of pre-B/immature B cells (CD43⁻B220^lo), pro-B cells (B220⁺CD43⁺), and mature recirculating B cells (B220^hiCD43⁻). (f) Individual values for each mouse tested of percentages of different B-cell precursors populations depicted in (e), in bone marrows obtained from B6 mice n=7, littermate CD21 cre mice n=4, littermate Mb1 cre mice n=7, CD21 cre mice n=7, and Mb1 cre mice n=8. Means and S.D. are indicated. *P<0.05, **P<0.01 significant after Mann–Whitney U-test