Figure 1.

FH gets actively internalized by various cell types. (a and b) Protein internalization by apoptotic Jurkat T cells incubated for 2, 10 and 30 min with 125 μg/ml pHrodo-labeled FH and 25 μg/ml protein S at 37 °C (a) and 4 °C (b) assessed by flow cytometry. (c) FH and protein S internalization by apoptotic RPE cells as in a. (d) FH internalization by early (annexin A5 positive) and late apoptotic (annexin A5 and Via-Probe positive) cell populations of Jurkat T cells. (a–d) Representative histograms of n=3 independent experiments. (e) Localization of 30 μg/ml AlexaFluor488-labeled FH (green) in apoptotic Jurkat T cells after 5 and 30 min incubation at 37 °C as well as 30 min incubation after pre-treatment with 30 mM NaN₃ assessed by confocal microscopy. Nuclei counterstained with propidium iodine (red). Representative images of n=3 independent experiments. Scale bars, 50 and 10 μm for zoomed images