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. 2015 Nov 17;34(1):55–66. doi: 10.1002/stem.2219

Figure 5.

Figure 5

lncRNA‐Hh promotes breast cancer tumorigenesis in nude mice. (A): Photos of the tumor‐bearing BALB/c female nude mice and the xenograft tumors dissected from the nude mice at 5 weeks after being subcutaneously injected with MCF‐7/lncRNA‐Hh, MDA‐MB‐231/shRNA‐lncRNA‐Hh mammosphere cells and their control mammosphere cells. (B): Tumor growth curve. The data were generated from three mice in four different groups described in (A). Data are the means ± SD of three independent experiments performed in triplicate (*, p ≤ .05; **, p ≤ .01). (C): Quantitative real‐time polymerase chain reaction was used to test levels of lncRNA‐Hh in MDA‐MB‐231‐sh/lncRNA‐Hh tumor tissue and MCF‐7/lncRNA‐Hh tumor tissue and their control tumor tissues. (D): Immunoblotting analysis of GAS1, GLI1, SOX2, and OCT4 in the tumor tissues described in (C). β‐Actin was used as a loading control. Data are the means ± SD of three independent experiments in (C) and (D). (E): Representative photomicrographs of tumor tissue sections stained with SOX2‐specific polyclonal antibody (magnification of 400×). Scale bars = 100 μm. Abbreviations: GAS1, growth arrest‐specific 1; lncRNA‐Hh, hedgehog pathway associated long noncoding RNA.