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. 2016 Apr 15;6:24324. doi: 10.1038/srep24324

Figure 3. Cancer cells reveal rhythmic expression of PFKFB3 and core clock genes.

Figure 3

Human tongue cancer SCC9 cells were used to measure 24 hr oscillation of the expression of PFKFB3, as well as core clock genes. After synchronization, cells were incubated for an additional 1, 5, 9, 13, 17, and/or 21 hr (zeitgeber time, ZT1, ZT5, ZT9, ZT13, ZT17, and/or ZT21) and harvested for preparation of total RNA. The mRNA levels of PFKFB3, as well as core clock genes were quantified using real-time PCR and plotted as relative expression. Data are means ± SE. (A) PFKFB3 oscillation. (B) BMAL1 oscillation. (C) CLOCK oscillation. (D) PER1 oscillation. (E) PER2 oscillation. Data are means ± SE. n = 6. *P < 0.05 and **P < 0.01 vs. ZT1 for increased expression of the same gene; †P < 0.05 and ††P < 0.01 vs. ZT1 for decreased expression of the same gene.