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. 2016 Apr 15;6:24371. doi: 10.1038/srep24371

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Copyright © 2016, Macmillan Publishers Limited

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Co-localization of endogenous TNF-α with different intracellular organelle markers in THP-1 cells with control shRNA or Sirt6 shRNA. (A) Confocal imaging of endogenous TNF-α, Rab11 (recycling endosome marker), Rab7 (late endosome marker), EEA1 (early endosome marker), and LAMP1 (lysosome marker) in THP-1 cells. (B) Western blot of endogenous SIRT6 in THP-1 cells with control shRNA or Sirt6 shRNA. (C) Overlap coefficients of TNF-α with Rab11, Rab7, EEA1, and LAMP1. Co-localization was quantified using coloc 2 plugin in ImageJ. Each bar is the mean ± SD (error bars) for three images. Two asterisks indicate significance with p < 0.01.